QARS Antibody

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ProSci
Product Code: PSI-22-587
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-587-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using QARS antibody (22-587) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
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Immunohistochemistry of paraffin-embedded human colon carcinoma using QARS antibody (22-587) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human liver damage using QARS antibody (22-587) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human gastric cancer using QARS antibody (22-587) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of U2OS cells using QARS antibody (22-587). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using QARS antibody (22-587) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Immunohistochemistry of paraffin-embedded human colon carcinoma using QARS antibody (22-587) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver damage using QARS antibody (22-587) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using QARS antibody (22-587) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using QARS antibody (22-587). Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
GLNRS, MSCCA, PRO2195, glutamine--tRNA ligase, glutamine-tRNA synthetase
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:100
Background:
Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. In metazoans, 9 aminoacyl-tRNA synthetases specific for glutamine (gln), glutamic acid (glu), and 7 other amino acids are associated within a multienzyme complex. Although present in eukaryotes, glutaminyl-tRNA synthetase (QARS) is absent from many prokaryotes, mitochondria, and chloroplasts, in which Gln-tRNA(Gln) is formed by transamidation of the misacylated Glu-tRNA(Gln). Glutaminyl-tRNA synthetase belongs to the class-I aminoacyl-tRNA synthetase family. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human QARS (NP_005042.1).
NCBI Gene ID #:
5859
NCBI Official Name:
glutaminyl-tRNA synthetase
NCBI Official Symbol:
QARS
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 88kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
P47897
User NOte:
Optimal dilutions for each application to be determined by the researcher.