RARRES2 Antibody

ProSci
Product Code: PSI-22-590
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-590-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using RARRES2 antibody (22-590) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 15min.
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Immunohistochemistry of paraffin-embedded rat kidney using RARRES2 antibody (22-590) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded human liver cancer using RARRES2 antibody (22-590) at dilution of 1:200 (40x lens).
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Immunofluorescence analysis of MCF-7 cells using RARRES2 antibody (22-590). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using RARRES2 antibody (22-590) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 15min.
Immunohistochemistry of paraffin-embedded rat kidney using RARRES2 antibody (22-590) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using RARRES2 antibody (22-590) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of MCF-7 cells using RARRES2 antibody (22-590). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
HP10433, TIG2, retinoic acid receptor responder protein 2, RAR-responsive protein TIG2, chemerin, retinoic acid receptor responder (tazarotene induced) 2, tazarotene-induced gene 2 protein
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:100
Background:
This gene encodes a secreted chemotactic protein that initiates chemotaxis via the ChemR23 G protein-coupled seven-transmembrane domain ligand. Expression of this gene is upregulated by the synthetic retinoid tazarotene and occurs in a wide variety of tissues. The active protein has several roles, including that as an adipokine and as an antimicrobial protein with activity against bacteria and fungi.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 17-163 of human RARRES2 (NP_002880.1).
NCBI Gene ID #:
5919
NCBI Official Name:
retinoic acid receptor responder 2
NCBI Official Symbol:
RARRES2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 18kDa
Purification:
Affinity purification
Research Area:
Cancer, Signal Transduction
Swissprot #:
Q99969
User NOte:
Optimal dilutions for each application to be determined by the researcher.