RBBP6 Antibody

ProSci
Product Code: PSI-22-593
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-593-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using RBBP6 antibody (22-593) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunofluorescence analysis of A-549 cells using RBBP6 antibody (22-593). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using RBBP6 antibody (22-593) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunofluorescence analysis of A-549 cells using RBBP6 antibody (22-593). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
MY038, P2P-R, PACT, RBQ-1, SNAMA, E3 ubiquitin-protein ligase RBBP6, RB-binding Q-protein 1, RING-type E3 ubiquitin transferase RBBP6, p53-associated cellular protein of testis, proliferation potential-related protein, protein P2P-R, retinoblastoma binding protein 6, retinoblastoma-binding Q protein 1, retinoblastoma-binding protein 6
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200
Background:
The retinoblastoma tumor suppressor (pRB) protein binds with many other proteins. In various human cancers, pRB suppresses cellular proliferation and is inactivated. Cell cycle-dependent phosphorylation regulates the activity of pRB. This gene encodes a protein which binds to underphosphorylated but not phosphorylated pRB. Multiple alternatively spliced transcript variants that encode different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-118 of human RBBP6 (NP_116015.2).
NCBI Gene ID #:
5930
NCBI Official Name:
RB binding protein 6, ubiquitin ligase
NCBI Official Symbol:
RBBP6
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 160kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cancer, Cell Cycle
Swissprot #:
Q7Z6E9
User NOte:
Optimal dilutions for each application to be determined by the researcher.