SGCD Antibody

ProSci
Product Code: PSI-22-603
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-603-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using SGCD antibody (22-603) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 2
Immunohistochemistry of paraffin-embedded rat heart using SGCD antibody (22-603) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using SGCD antibody (22-603) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat heart using SGCD antibody (22-603) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
dystrophin associated, delta-sarcoglycan, delta-SG, 35kD dystrophin-associated glycoprotein, SGD, SGCDP, SG-delta, MGC22567, DAGD, CMD1L, 35DAG, sarcoglycan, delta (35kDa dystrophin-associated glycoprotein), SGCD
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:100
Background:
The protein encoded by this gene is one of the four known components of the sarcoglycan complex, which is a subcomplex of the dystrophin-glycoprotein complex (DGC). DGC forms a link between the F-actin cytoskeleton and the extracellular matrix. This protein is expressed most abundantly in skeletal and cardiac muscle. Mutations in this gene have been associated with autosomal recessive limb-girdle muscular dystrophy and dilated cardiomyopathy. Alternatively spliced transcript variants encoding distinct isoforms have been observed for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 61-290 of human SGCD (NP_000328.2).
NCBI Gene ID #:
6444
NCBI Official Name:
sarcoglycan, delta (35kDa dystrophin-associated glycoprotein)
NCBI Official Symbol:
SGCD
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 37kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Signal Transduction
Swissprot #:
Q92629
User NOte:
Optimal dilutions for each application to be determined by the researcher.