SOD3 Antibody

ProSci
Product Code: PSI-22-606
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-606-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using SOD3 antibody (22-606) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
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Immunohistochemistry of paraffin-embedded human mammary cancer using SOD3 antibody (22-606) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat ovary using SOD3 antibody (22-606) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse pancreas using SOD3 antibody (22-606) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using SOD3 antibody (22-606) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human mammary cancer using SOD3 antibody (22-606) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat ovary using SOD3 antibody (22-606) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse pancreas using SOD3 antibody (22-606) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
SOD3, EC-SOD
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes a member of the superoxide dismutase (SOD) protein family. SODs are antioxidant enzymes that catalyze the conversion of superoxide radicals into hydrogen peroxide and oxygen, which may protect the brain, lungs, and other tissues from oxidative stress. Proteolytic processing of the encoded protein results in the formation of two distinct homotetramers that differ in their ability to interact with the extracellular matrix (ECM). Homotetramers consisting of the intact protein, or type C subunit, exhibit high affinity for heparin and are anchored to the ECM. Homotetramers consisting of a proteolytically cleaved form of the protein, or type A subunit, exhibit low affinity for heparin and do not interact with the ECM. A mutation in this gene may be associated with increased heart disease risk.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 19-160 of human SOD3 (NP_003093.2).
NCBI Gene ID #:
6649
NCBI Official Name:
superoxide dismutase 3, extracellular
NCBI Official Symbol:
SOD3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 35kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle
Swissprot #:
P08294
User NOte:
Optimal dilutions for each application to be determined by the researcher.