SOX5 Antibody

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ProSci
Product Code: PSI-22-607
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-607-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using SOX5 antibody (22-607) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 3s.
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Immunohistochemistry of paraffin-embedded rat kidney using SOX5 antibody (22-607) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human colon carcinoma using SOX5 antibody (22-607) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human kidney cancer using SOX5 antibody (22-607) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human stomach using SOX5 antibody (22-607) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using SOX5 antibody (22-607) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of MCF7 cells using SOX5 antibody (22-607).
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Western blot analysis of extracts of various cell lines, using SOX5 antibody (22-607) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 3s.
Immunohistochemistry of paraffin-embedded rat kidney using SOX5 antibody (22-607) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using SOX5 antibody (22-607) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney cancer using SOX5 antibody (22-607) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using SOX5 antibody (22-607) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using SOX5 antibody (22-607) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF7 cells using SOX5 antibody (22-607).

Documents

Further Information

Additional Names:
SOX5, L-SOX5, L-SOX5B, L-SOX5F, RP11-444D3.1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:100
Background:
This gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins. The encoded protein may play a role in chondrogenesis. A pseudogene of this gene is located on chromosome 8. Multiple transcript variants encoding distinct isoforms have been identified for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human SOX5 (NP_694534.1).
NCBI Gene ID #:
6660
NCBI Official Name:
SRY (sex determining region Y)-box 5
NCBI Official Symbol:
SOX5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 70kDa
Purification:
Affinity purification
Research Area:
Transcription
Swissprot #:
P35711
User NOte:
Optimal dilutions for each application to be determined by the researcher.