STK3 Antibody

ProSci
Product Code: PSI-22-614
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-614-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using STK3 antibody (22-614) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
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Immunohistochemistry of paraffin-embedded human prostate using STK3 antibody (22-614) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human stomach using STK3 antibody (22-614) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat kidney using STK3 antibody (22-614) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse stomach using STK3 antibody (22-614) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of NIH-3T3 cells using STK3 Polyclonal Antibody (22-614) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using STK3 antibody (22-614) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
Immunohistochemistry of paraffin-embedded human prostate using STK3 antibody (22-614) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using STK3 antibody (22-614) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using STK3 antibody (22-614) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse stomach using STK3 antibody (22-614) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of NIH-3T3 cells using STK3 Polyclonal Antibody (22-614) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
STK3, FLJ90748, KRS1, MST2
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:100
Background:
This gene encodes a serine/threonine protein kinase activated by proapoptotic molecules indicating the encoded protein functions as a growth suppressor. Cleavage of the protein product by caspase removes the inhibitory C-terminal portion. The N-terminal portion is transported to the nucleus where it homodimerizes to form the active kinase which promotes the condensation of chromatin during apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 323-450 of human STK3 (NP_006272.2).
NCBI Gene ID #:
6788
NCBI Official Name:
serine/threonine kinase 3
NCBI Official Symbol:
STK3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 65kDa, 37kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Autophagy, Cell Cycle, Signal Transduction
Swissprot #:
Q13188
User NOte:
Optimal dilutions for each application to be determined by the researcher.