WRB Antibody

ProSci
Product Code: PSI-22-621
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-621-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using WRB antibody (22-621) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 2
Immunohistochemistry of paraffin-embedded rat kidney using WRB antibody (22-621) at dilution of 1:200 (40x lens).

Western blot analysis of extracts of various cell lines, using WRB antibody (22-621) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat kidney using WRB antibody (22-621) at dilution of 1:200 (40x lens).

Further Information

Additional Names:
CHD5, Tail-anchored protein insertion receptor WRB, Congenital heart disease 5 protein, WRB
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
This gene encodes a basic nuclear protein of unknown function. The gene is widely expressed in adult and fetal tissues. Since the region proposed to contain the gene(s) for congenital heart disease (CHD) in Down syndrome (DS) patients has been restricted to 21q22.2-22.3, this gene, which maps to 21q22.3, has a potential role in the pathogenesis of Down syndrome congenital heart disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 30-100 of human WRB (NP_004618.2).
NCBI Gene ID #:
7485
NCBI Official Name:
tryptophan rich basic protein
NCBI Official Symbol:
WRB
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 25kDa
Purification:
Affinity purification
Research Area:
Neuroscience
Swissprot #:
O00258
User NOte:
Optimal dilutions for each application to be determined by the researcher.