ZNF148 Antibody

ProSci
Product Code: PSI-22-622
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-622-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using ZNF148 Antibody (22-622) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
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Immunohistochemistry of paraffin-embedded rat lung using ZNF148 antibody (22-622) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat liver using ZNF148 antibody (22-622) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat brain using ZNF148 antibody (22-622) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat heart using ZNF148 antibody (22-622) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human lung using ZNF148 antibody (22-622) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human colon using ZNF148 antibody (22-622) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human appendix using ZNF148 antibody (22-622) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human smooth muscle using ZNF148 antibody (22-622) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human gastric cancer using NASP Antibody (22-569) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse liver using ZNF148 antibody (22-622) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using ZNF148 antibody (22-622) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse kidney using ZNF148 antibody (22-622) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse heart using ZNF148 antibody (22-622) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of A-549 cells using ZNF148 antibody (22-622).

Western blot analysis of extracts of various cell lines, using ZNF148 Antibody (22-622) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
Immunohistochemistry of paraffin-embedded rat lung using ZNF148 antibody (22-622) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat liver using ZNF148 antibody (22-622) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using ZNF148 antibody (22-622) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using ZNF148 antibody (22-622) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung using ZNF148 antibody (22-622) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using ZNF148 antibody (22-622) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human appendix using ZNF148 antibody (22-622) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human smooth muscle using ZNF148 antibody (22-622) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using NASP Antibody (22-569) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using ZNF148 antibody (22-622) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using ZNF148 antibody (22-622) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using ZNF148 antibody (22-622) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using ZNF148 antibody (22-622) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A-549 cells using ZNF148 antibody (22-622).

Further Information

Additional Names:
Zinc finger protein 148, Transcription factor ZBP-89, Zinc finger DNA-binding protein 89, ZNF148, ZBP89
Application Note:
WB: 1:500 - 1:1000

IHC: 1:50 - 1:100

IF: 1:20 - 1:100
Background:
The protein encoded by this gene is a member of the Kruppel family of zinc finger DNA binding proteins. The encoded protein activates transcription of the T-cell receptor and intestinal alkaline phosphatase genes but represses transcription of the ornithine decarboxylase, vimentin, gastrin, stomelysin, and enolase genes. Increased expression of this gene results in decreased patient survival rates from colorectal cancer, while mutations in this gene have been associated with global developmental delay, hypoplastic corpus callosum, and dysmorphic facies.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 690-794 of human ZNF148 (NP_068799.2).
NCBI Gene ID #:
7707
NCBI Official Name:
Zinc finger protein 148
NCBI Official Symbol:
ZNF148
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 105kDa
Purification:
Affinity purification
Research Area:
Transcription
Swissprot #:
Q9UQR1
User NOte:
Optimal dilutions for each application to be determined by the researcher.