NAA10 Antibody

ProSci
Product Code: PSI-22-628
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-628-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using NAA10 antibody (22-628) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
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Immunohistochemistry of paraffin-embedded rat lung using NAA10 antibody (22-628) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human breast cancer using NAA10 antibody (22-628) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human stomach using NAA10 antibody (22-628) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse lung using NAA10 antibody (22-628) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of U2OS cells using NAA10 antibody (22-628). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using NAA10 antibody (22-628) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Immunohistochemistry of paraffin-embedded rat lung using NAA10 antibody (22-628) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using NAA10 antibody (22-628) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using NAA10 antibody (22-628) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using NAA10 antibody (22-628) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using NAA10 antibody (22-628). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
ARD1A, ARD1, DXS707, MGC71248, TE2, NATD, ARD1A, ARD1P, MCOPS1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:100
Background:
N-alpha-acetylation is among the most common post-translational protein modifications in eukaryotic cells. This process involves the transfer of an acetyl group from acetyl-coenzyme A to the alpha-amino group on a nascent polypeptide and is essential for normal cell function. This gene encodes an N-terminal acetyltransferase that functions as the catalytic subunit of the major amino-terminal acetyltransferase A complex. Mutations in this gene are the cause of Ogden syndrome. Alternate splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 161-235 of human NAA10 (NP_003482.1).
NCBI Gene ID #:
8260
NCBI Official Name:
N(alpha)-acetyltransferase 10, NatA catalytic subunit
NCBI Official Symbol:
NAA10
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 26kDa
Purification:
Affinity purification
Research Area:
Signal Transduction
Swissprot #:
P41227
User NOte:
Optimal dilutions for each application to be determined by the researcher.