HIRIP3 Antibody

ProSci
Product Code: PSI-22-631
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-631-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using HIRIP3 antibody (22-631) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded rat liver using HIRIP3 antibody (22-631) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat spleen using HIRIP3 antibody (22-631) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human liver cancer using HIRIP3 antibody (22-631) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human oophoroma using HIRIP3 antibody (22-631) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human well-differentiated squamous skin carcinoma using HIRIP3 antibody (22-631) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human kidney using HIRIP3 antibody (22-631) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human kidney cancer using HIRIP3 antibody (22-631) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse liver using HIRIP3 antibody (22-631) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse lung using ZNF148 antibody (22-622) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of U2OS cells using HIRIP3 antibody (22-631).

Western blot analysis of extracts of various cell lines, using HIRIP3 antibody (22-631) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat liver using HIRIP3 antibody (22-631) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat spleen using HIRIP3 antibody (22-631) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using HIRIP3 antibody (22-631) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human oophoroma using HIRIP3 antibody (22-631) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human well-differentiated squamous skin carcinoma using HIRIP3 antibody (22-631) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney using HIRIP3 antibody (22-631) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney cancer using HIRIP3 antibody (22-631) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using HIRIP3 antibody (22-631) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using ZNF148 antibody (22-622) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using HIRIP3 antibody (22-631).

Further Information

Additional Names:
HIRA-interacting protein 3, HIRIP3
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:100
Background:
The HIRA protein shares sequence similarity with Hir1p and Hir2p, the two corepressors of histone gene transcription characterized in the yeast, Saccharomyces cerevisiae. The structural features of the HIRA protein suggest that it may function as part of a multiprotein complex. Several cDNAs encoding HIRA-interacting proteins, or HIRIPs, have been identified. In vitro, the protein encoded by this gene binds HIRA, as well as H2B and H3 core histones, indicating that a complex containing HIRA-HIRIP3 could function in some aspects of chromatin and histone metabolism. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 417-556 of human HIRIP3 (NP_003600.2).
NCBI Gene ID #:
8479
NCBI Official Name:
HIRA-interacting protein 3
NCBI Official Symbol:
HIRIP3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 62kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Stem Cell
Swissprot #:
Q9BW71
User NOte:
Optimal dilutions for each application to be determined by the researcher.