EIF2B2 Antibody

ProSci
Product Code: PSI-22-641
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-641-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using EIF2B2 antibody (22-641) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
2 / 2
Immunohistochemistry of paraffin-embedded human stomach using EIF2B2 antibody (22-641) at dilution of 1:200 (40x lens).

Western blot analysis of extracts of various cell lines, using EIF2B2 antibody (22-641) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Immunohistochemistry of paraffin-embedded human stomach using EIF2B2 antibody (22-641) at dilution of 1:200 (40x lens).

Further Information

Additional Names:
EIF2BB, Translation initiation factor eIF-2B subunit beta, S20I15, EIF2B, EIF-2Bbeta, Translation initiation factor eIF2B b subunit, eIF-2B GDP-GTP exchange factor, S20III15
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200

IP: 1:50 - 1:200
Background:
This gene encodes the beta subunit of eukaryotic initiation factor-2B (EIF2B). EIF2B is involved in protein synthesis and exchanges GDP and GTP for its activation and deactivation.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-351 of human EIF2B2 (NP_055054.1).
NCBI Gene ID #:
8892
NCBI Official Name:
eukaryotic translation initiation factor 2B, subunit 2 beta, 39kDa
NCBI Official Symbol:
EIF2B2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 39kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
P49770
User NOte:
Optimal dilutions for each application to be determined by the researcher.