DNAJA3 Antibody

ProSci
Product Code: PSI-22-642
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-642-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using DNAJA3 antibody (22-642) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
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Immunohistochemistry of paraffin-embedded rat brain using DNAJA3 Antibody (22-642) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat kidney using DNAJA3 Antibody (22-642) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using DNAJA3 antibody (22-642) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Immunohistochemistry of paraffin-embedded rat brain using DNAJA3 Antibody (22-642) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using DNAJA3 Antibody (22-642) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
DNAJA3, DnaJ protein Tid-1, HTID-1, HCA57, TID1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
This gene encodes a member of the DNAJ/Hsp40 protein family. DNAJ/Hsp40 proteins stimulate the ATPase activity of Hsp70 chaperones and play critical roles in protein folding, degradation, and multimeric complex assembly. The encoded protein is localized to mitochondria and mediates several cellular processes including proliferation, survival and apoptotic signal transduction. The encoded protein also plays a critical role in tumor suppression through interactions with oncogenic proteins including ErbB2 and the p53 tumor suppressor protein. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 221-480 of human DNAJA3 (NP_005138.3).
NCBI Gene ID #:
9093
NCBI Official Name:
DnaJ (Hsp40) homolog, subfamily A, member 3
NCBI Official Symbol:
DNAJA3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 52kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cell Cycle, Signal Transduction
Swissprot #:
Q96EY1
User NOte:
Optimal dilutions for each application to be determined by the researcher.