DDX21 Antibody

ProSci
Product Code: PSI-22-646
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-646-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using DDX21 antibody (22-646) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 60s.
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Immunohistochemistry of paraffin-embedded human kidney cancer using DDX21 antibody (22-646) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using DDX21 antibody (22-646) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 60s.
Immunohistochemistry of paraffin-embedded human kidney cancer using DDX21 antibody (22-646) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
DDX21, GUA, GURDB, RH-II/GU, RH-II/GuA
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is an antigen recognized by autoimmune antibodies from a patient with watermelon stomach disease. This protein unwinds double-stranded RNA, folds single-stranded RNA, and may play important roles in ribosomal RNA biogenesis, RNA editing, RNA transport, and general transcription.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 624-783 of human DDX21 (NP_004719.2).
NCBI Gene ID #:
9188
NCBI Official Name:
DEAD (Asp-Glu-Ala-Asp) box helicase 21
NCBI Official Symbol:
DDX21
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 100kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
Q9NR30
User NOte:
Optimal dilutions for each application to be determined by the researcher.