EFTUD2 Antibody

ProSci
Product Code: PSI-22-651
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-651-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using EFTUD2 antibody (22-651) at 1:2000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 20s.
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Immunohistochemistry of paraffin-embedded rat liver using EFTUD2 Antibody (22-651) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human stomach using EFTUD2 Antibody (22-651) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human gastric cancer using EFTUD2 Antibody (22-651) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using EFTUD2 Antibody (22-651) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse kidney using EFTUD2 Antibody (22-651) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of U2OS cells using EFTUD2 antibody (22-651).
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Immunoprecipitation analysis of 100ug extracts of 293T cells using 3ug EFTUD2 antibody (22-651). Western blot was performed from the immunoprecipitate using EFTUD2 antibody (22-651) at a dilition of 1:1000.

Western blot analysis of extracts of various cell lines, using EFTUD2 antibody (22-651) at 1:2000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 20s.
Immunohistochemistry of paraffin-embedded rat liver using EFTUD2 Antibody (22-651) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using EFTUD2 Antibody (22-651) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using EFTUD2 Antibody (22-651) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using EFTUD2 Antibody (22-651) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using EFTUD2 Antibody (22-651) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using EFTUD2 antibody (22-651).
Immunoprecipitation analysis of 100ug extracts of 293T cells using 3ug EFTUD2 antibody (22-651). Western blot was performed from the immunoprecipitate using EFTUD2 antibody (22-651) at a dilition of 1:1000.

Further Information

Additional Names:
116 kDa U5 small nuclear ribonucleoprotein component, Elongation factor Tu GTP-binding domain-containing protein 2, SNU114 homolog, hSNU114, U5 snRNP-specific protein, 116 kDa, U5-116 kDa, EFTUD2, KIAA0031, SNRP116
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:100

IP: 1:20 - 1:50
Background:
This gene encodes a GTPase which is a component of the spliceosome complex which processes precursor mRNAs to produce mature mRNAs. Mutations in this gene are associated with mandibulofacial dysostosis with microcephaly. Multiple transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-205 of human EFTUD2 (NP_004238.3).
NCBI Gene ID #:
9343
NCBI Official Name:
116 kDa U5 small nuclear ribonucleoprotein component
NCBI Official Symbol:
EFTUD2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 120kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
Q15029
User NOte:
Optimal dilutions for each application to be determined by the researcher.