NMT2 Antibody

ProSci
Product Code: PSI-22-653
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-653-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using NMT2 antibody (22-653) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
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Immunohistochemistry of paraffin-embedded mouse kidney using NMT2 antibody (22-653) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of A-549 cells using NMT2 antibody (22-653). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using NMT2 antibody (22-653) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Immunohistochemistry of paraffin-embedded mouse kidney using NMT2 antibody (22-653) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A-549 cells using NMT2 antibody (22-653). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
Glycylpeptide N-tetradecanoyltransferase 2, Myristoyl-CoA:protein N-myristoyltransferase 2, NMT 2, Peptide N-myristoyltransferase 2, Type II N-myristoyltransferase, NMT2
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:100
Background:
This gene encodes one of two N-myristoyltransferase proteins. N-terminal myristoylation is a lipid modification that is involved in regulating the function and localization of signaling proteins. The encoded protein catalyzes the addition of a myristoyl group to the N-terminal glycine residue of many signaling proteins, including the human immunodeficiency virus type 1 (HIV-1) proteins, Gag and Nef. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human NMT2 (NP_004799.1).
NCBI Gene ID #:
9397
NCBI Official Name:
Glycylpeptide N-tetradecanoyltransferase 2
NCBI Official Symbol:
NMT2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 57kDa
Purification:
Affinity purification
Research Area:
Cancer, Signal Transduction
Swissprot #:
O60551
User NOte:
Optimal dilutions for each application to be determined by the researcher.