HAND2 Antibody

ProSci
Product Code: PSI-22-655
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-655-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using HAND2 antibody (22-655) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunofluorescence analysis of A549 cells using HAND2 antibody (22-655). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using HAND2 antibody (22-655) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunofluorescence analysis of A549 cells using HAND2 antibody (22-655). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
HAND2, DHand, DHAND2, Hed, Thing2, BHLHa26
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:100
Background:
The protein encoded by this gene belongs to the basic helix-loop-helix family of transcription factors. This gene product is one of two closely related family members, the HAND proteins, which are asymmetrically expressed in the developing ventricular chambers and play an essential role in cardiac morphogenesis. Working in a complementary fashion, they function in the formation of the right ventricle and aortic arch arteries, implicating them as mediators of congenital heart disease. In addition, this transcription factor plays an important role in limb and branchial arch development.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-217 of human HAND2 (NP_068808.1).
NCBI Gene ID #:
9464
NCBI Official Name:
heart and neural crest derivatives expressed 2
NCBI Official Symbol:
HAND2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 26kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cell Cycle, Neuroscience, Transcription
Swissprot #:
P61296
User NOte:
Optimal dilutions for each application to be determined by the researcher.