DNAJB6 Antibody

ProSci
Product Code: PSI-22-660
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-660-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using DNAJB6 antibody (22-660) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
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Immunohistochemistry of paraffin-embedded human well-differentiated squamous skin carcinoma using DNAJB6 antibody (22-660) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human kidney using DNAJB6 antibody (22-660) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human kidney cancer using DNAJB6 antibody (22-660) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human stomach using DNAJB6 antibody (22-660) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse ileum using DNAJB6 antibody (22-660) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of U2OS cells using DNAJB6 antibody (22-660). Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of HeLa cells using DNAJB6 antibody (22-660) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using DNAJB6 antibody (22-660) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Immunohistochemistry of paraffin-embedded human well-differentiated squamous skin carcinoma using DNAJB6 antibody (22-660) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney using DNAJB6 antibody (22-660) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney cancer using DNAJB6 antibody (22-660) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using DNAJB6 antibody (22-660) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse ileum using DNAJB6 antibody (22-660) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using DNAJB6 antibody (22-660). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using DNAJB6 antibody (22-660) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
DNAJB6, DKFZp566D0824, DnaJ, FLJ42837, HHDJ1, HSJ-2, HSJ2, MGC1152, MGC117297, MRJ, MSJ-1, DJ4, LGMD1D, LGMD1E
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:100
Background:
This gene encodes a member of the DNAJ protein family. DNAJ family members are characterized by a highly conserved amino acid stretch called the 'J-domain' and function as one of the two major classes of molecular chaperones involved in a wide range of cellular events, such as protein folding and oligomeric protein complex assembly. This family member may also play a role in polyglutamine aggregation in specific neurons. Alternative splicing of this gene results in multiple transcript variants; however, not all variants have been fully described.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 177-326 of human DNAJB6 (NP_490647.1).
NCBI Gene ID #:
10049
NCBI Official Name:
DnaJ (Hsp40) homolog, subfamily B, member 6
NCBI Official Symbol:
DNAJB6
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 40kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cell Cycle, Neuroscience, Signal Transduction
Swissprot #:
O75190
User NOte:
Optimal dilutions for each application to be determined by the researcher.