STAM2 Antibody

ProSci
Product Code: PSI-22-666
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-666-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using STAM2 antibody (22-666) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
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Immunofluorescence analysis of U2OS cells using STAM2 antibody (22-666). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using STAM2 antibody (22-666) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Immunofluorescence analysis of U2OS cells using STAM2 antibody (22-666). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
STAM2, signal transducing adaptor molecule (SH3 domain and ITAM motif) 2, HGNC:11358, DKFZp564C047, Hbp, STAM2A, STAM2B, STAM-like protein containing SH3 and ITAM domains 2, signal transducing adaptor molecule 2, HBP
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:100
Background:
The protein encoded by this gene is closely related to STAM, an adaptor protein involved in the downstream signaling of cytokine receptors, both of which contain a SH3 domain and the immunoreceptor tyrosine-based activation motif (ITAM). Similar to STAM, this protein acts downstream of JAK kinases, and is phosphorylated in response to cytokine stimulation. This protein and STAM thus are thought to exhibit compensatory effects on the signaling pathway downstream of JAK kinases upon cytokine stimulation.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 376-525 of human STAM2 (NP_005834.4).
NCBI Gene ID #:
10254
NCBI Official Name:
signal transducing adaptor molecule (SH3 domain and ITAM motif) 2
NCBI Official Symbol:
STAM2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 70kDa
Purification:
Affinity purification
Research Area:
Signal Transduction
Swissprot #:
O75886
User NOte:
Optimal dilutions for each application to be determined by the researcher.