PPIE Antibody

ProSci
Product Code: PSI-22-668
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-668-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 2
Western blot analysis of extracts of various cell lines, using PPIE antibody (22-668) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
2 / 2
Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug PPIE antibody (22-668). Western blot was performed from the immunoprecipitate using PPIE antibody (22-668) at a dilition of 1:1000.

Western blot analysis of extracts of various cell lines, using PPIE antibody (22-668) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug PPIE antibody (22-668). Western blot was performed from the immunoprecipitate using PPIE antibody (22-668) at a dilition of 1:1000.

Further Information

Additional Names:
PPIE, CYP33, CYP-33
Application Note:
WB: 1:500 - 1:2000

IP: 1:50 - 1:200
Background:
The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein contains a highly conserved cyclophilin (CYP) domain as well as an RNA-binding domain. It was shown to possess PPIase and protein folding activities, and it also exhibits RNA-binding activity. Alternative splicing results in multiple transcript variants. A related pseudogene, which is also located on chromosome 1, has been identified.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-301 of human PPIE (NP_006103.1).
NCBI Gene ID #:
10450
NCBI Official Name:
peptidylprolyl isomerase E (cyclophilin E)
NCBI Official Symbol:
PPIE
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 33kDa
Purification:
Affinity purification
Research Area:
Signal Transduction
Swissprot #:
Q9UNP9
User NOte:
Optimal dilutions for each application to be determined by the researcher.