OGFR Antibody

ProSci
Product Code: PSI-22-678
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-678-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using OGFR antibody (22-678) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded human stomach using OGFR antibody (22-678) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat kidney using OGFR antibody (22-678) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse stomach using OGFR antibody (22-678) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of MCF7 cells using OGFR antibody (22-678). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using OGFR antibody (22-678) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human stomach using OGFR antibody (22-678) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using OGFR antibody (22-678) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse stomach using OGFR antibody (22-678) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF7 cells using OGFR antibody (22-678). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
OGFR, opioid growth factor receptor, HGNC:15768, 7-60 protein, zeta-type opioid receptor, met-enkephalin receptor,
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:100
Background:
The protein encoded by this gene is a receptor for opioid growth factor (OGF), also known as [Met(5)]-enkephalin. OGF is a negative regulator of cell proliferation and tissue organization in a variety of processes. The encoded unbound receptor for OGF has been localized to the outer nuclear envelope, where it binds OGF and is translocated into the nucleus. The coding sequence of this gene contains a polymorphic region of 60 nt tandem imperfect repeat units. Several transcripts containing between zero and eight repeat units have been reported.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human OGFR (NP_031372.2).
NCBI Gene ID #:
11054
NCBI Official Name:
opioid growth factor receptor
NCBI Official Symbol:
OGFR
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 110kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Signal Transduction
Swissprot #:
Q9NZT2
User NOte:
Optimal dilutions for each application to be determined by the researcher.