CLIC4 Antibody

ProSci
Product Code: PSI-22-687
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-687-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 3
Western blot analysis of extracts of various cell lines, using CLIC4 antibody (22-687) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 2s.
2 / 3
Immunohistochemistry of paraffin-embedded rat brain using CLIC4 antibody (22-687) at dilution of 1:100 (40x lens).
3 / 3
Immunofluorescence analysis of A-549 cells using CLIC4 antibody (22-687). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using CLIC4 antibody (22-687) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 2s.
Immunohistochemistry of paraffin-embedded rat brain using CLIC4 antibody (22-687) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A-549 cells using CLIC4 antibody (22-687). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
CLIon Channel4, H1, huH1, p64H1, CLIC4L, MTCLIC
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:100
Background:
Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. Chloride intracellular channel 4 (CLIC4) protein, encoded by the CLIC4 gene, is a member of the p64 family; the gene is expressed in many tissues and exhibits a intracellular vesicular pattern in Panc-1 cells (pancreatic cancer cells).
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-253 of human CLIC4 (NP_039234.1).
NCBI Gene ID #:
25932
NCBI Official Name:
chloride intracellular channel 4
NCBI Official Symbol:
CLIC4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 29kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cancer, Cell Cycle, Signal Transduction
Swissprot #:
Q9Y696
User NOte:
Optimal dilutions for each application to be determined by the researcher.