LDLRAP1 Antibody

ProSci
Product Code: PSI-22-691
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-691-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of mouse liver, using LDLRAP1 antibody (22-691) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 4
Immunohistochemistry of paraffin-embedded rat brain using LDLRAP1 antibody (22-691) at dilution of 1:100 (40x lens).
3 / 4
Immunohistochemistry of paraffin-embedded rat kidney using LDLRAP1 antibody (22-691) at dilution of 1:100 (40x lens).
4 / 4
Immunofluorescence analysis of A-549 cells using LDLRAP1 antibody (22-691).

Western blot analysis of extracts of mouse liver, using LDLRAP1 antibody (22-691) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat brain using LDLRAP1 antibody (22-691) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using LDLRAP1 antibody (22-691) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A-549 cells using LDLRAP1 antibody (22-691).

Further Information

Additional Names:
ARH, ARH2, FHCB1, FHCB2, MGC34705, DKFZp586D0624, LDL receptor adaptor protein, low density lipoprotein receptor adaptor protein 1, ARH
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:100
Background:
The protein encoded by this gene is a cytosolic protein which contains a phosphotyrosine binding (PTD) domain. The PTD domain has been found to interact with the cytoplasmic tail of the LDL receptor. Mutations in this gene lead to LDL receptor malfunction and cause the disorder autosomal recessive hypercholesterolaemia.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 59-308 of human LDLRAP1 (NP_056442.2).
NCBI Gene ID #:
26119
NCBI Official Name:
low density lipoprotein receptor adaptor protein 1
NCBI Official Symbol:
LDLRAP1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 38kDa
Purification:
Affinity purification
Research Area:
Cancer, Signal Transduction
Swissprot #:
Q5SW96
User NOte:
Optimal dilutions for each application to be determined by the researcher.