TRMT2A Antibody

ProSci
Product Code: PSI-22-693
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-693-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using TRMT2A antibody (22-693) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 2
Immunohistochemistry of paraffin-embedded human lung cancer using TRMT2A antibody (22-693) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using TRMT2A antibody (22-693) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human lung cancer using TRMT2A antibody (22-693) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
HTF9C, tRNA (uracil-5-)-methyltransferase homolog A, TRM2 tRNA methyltransferase 2 homolog A, hpaII tiny fragments locus 9c protein
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
The protein encoded by this gene is of unknown function. However, it is orthologous to the mouse Trmt2a gene and contains an RNA methyltransferase domain. Expression of this gene varies during the cell cycle, with aberrant expression being a possible biomarker in certain breast cancers. Several transcript variants encoding two different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human TRMT2A (NP_073564.3).
NCBI Gene ID #:
27037
NCBI Official Name:
tRNA methyltransferase 2 homolog A
NCBI Official Symbol:
TRMT2A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 75kDa
Purification:
Affinity purification
Research Area:
Cancer
Swissprot #:
Q8IZ69
User NOte:
Optimal dilutions for each application to be determined by the researcher.