VPS4A Antibody

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ProSci
Product Code: PSI-22-694
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-694-50uL50uL£433.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using VPS4A antibody (22-694) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
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Immunohistochemistry of paraffin-embedded human stomach using VPS4A antibody (22-694) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human lung using VPS4A antibody (22-694) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of various cell lines, using VPS4A antibody (22-694) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human stomach using VPS4A antibody (22-694) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung using VPS4A antibody (22-694) at dilution of 1:100 (40x lens).

Documents

Further Information

Additional Names:
VPS4A, FLJ22197, SKD1, SKD2, VPS4, VPS4-1, SKD1A
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100
Background:
The protein encoded by this gene is a member of the AAA protein family (ATPases associated with diverse cellular activities), and is the homolog of the yeast Vps4 protein. In humans, two paralogs of the yeast protein have been identified. The former share a high degree of aa sequence similarity with each other, and also with yeast Vps4 and mouse Skd1 proteins. The mouse Skd1 (suppressor of K+ transport defect 1) has been shown to be really an yeast Vps4 ortholog. Functional studies indicate that both human paralogs associate with the endosomal compartments, and are involved in intracellular protein trafficking, similar to Vps4 protein in yeast. The gene encoding this paralog has been mapped to chromosome 16; the gene for the other resides on chromosome 18.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human VPS4A (NP_037377.1).
NCBI Gene ID #:
27183
NCBI Official Name:
vacuolar protein sorting 4 homolog A (S. cerevisiae)
NCBI Official Symbol:
VPS4A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 49kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Chemokines & Cytokines, Immunology, Signal Transduction
Swissprot #:
Q9UN37
User NOte:
Optimal dilutions for each application to be determined by the researcher.