NDE1 Antibody

ProSci
Product Code: PSI-22-709
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-709-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using NDE1 antibody (22-709) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded rat brain using NDE1 antibody (22-709) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat spleen using NDE1 antibody (22-709) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human breast cancer using NDE1 antibody (22-709) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using NDE1 antibody (22-709) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat brain using NDE1 antibody (22-709) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat spleen using NDE1 antibody (22-709) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using NDE1 antibody (22-709) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
NDE1, FLJ20101, HOM-TES-87, NUDE, NUDE1, NDE, LIS4
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
This gene encodes a member of the nuclear distribution E (NudE) family of proteins. The encoded protein is localized at the centrosome and interacts with other centrosome components as part of a multiprotein complex that regulates dynein function. This protein plays an essential role in microtubule organization, mitosis and neuronal migration. Mutations in this gene cause lissencephaly 4, a disorder characterized by lissencephaly, severe brain atrophy, microcephaly, and severe mental retardation. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-110 of human NDE1 (NP_060138.1).
NCBI Gene ID #:
54820
NCBI Official Name:
nudE neurodevelopment protein 1
NCBI Official Symbol:
NDE1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 39kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Neuroscience
Swissprot #:
Q9NXR1
User NOte:
Optimal dilutions for each application to be determined by the researcher.