NDUFAF5 Antibody

ProSci
Product Code: PSI-22-730
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-730-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using NDUFAF5 antibody (22-730) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.

Western blot analysis of extracts of various cell lines, using NDUFAF5 antibody (22-730) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.

Further Information

Additional Names:
C20orf7, MC1DN16, bA526K24.2, dJ842G6.1, arginine-hydroxylase NDUFAF5, mitochondrial, NADH dehydrogenase (ubiquinone) complex I, assembly factor 5, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 5, probable methyltransferase C20orf7, mitochondrial, putative methyltransferase NDUFAF5
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
The NADH-ubiquinone oxidoreductase complex (complex I) of the mitochondrial respiratory chain catalyzes the transfer of electrons from NADH to ubiquinone, and consists of at least 43 subunits. The complex is located in the inner mitochondrial membrane. This gene encodes a mitochondrial protein that is associated with the matrix face of the mitochondrial inner membrane and is required for complex I assembly. A mutation in this gene results in mitochondrial complex I deficiency. Multiple transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 196-345 of human NDUFAF5 (NP_077025.2).
NCBI Gene ID #:
79133
NCBI Official Name:
NADH:ubiquinone oxidoreductase complex assembly factor 5
NCBI Official Symbol:
NDUFAF5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 39kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
Q5TEU4
User NOte:
Optimal dilutions for each application to be determined by the researcher.