PTGES2 Antibody

ProSci
Product Code: PSI-22-732
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-732-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using PTGES2 antibody (22-732) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
2 / 5
Immunohistochemistry of paraffin-embedded rat kidney using PTGES2 antibody (22-732) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human kidney cancer using PTGES2 antibody (22-732) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse kidney using PTGES2 antibody (22-732) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of A-549 cells using PTGES2 antibody (22-732). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using PTGES2 antibody (22-732) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
Immunohistochemistry of paraffin-embedded rat kidney using PTGES2 antibody (22-732) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney cancer using PTGES2 antibody (22-732) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using PTGES2 antibody (22-732) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A-549 cells using PTGES2 antibody (22-732). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
C9orf15, GBF-1, GBF1, PGES2, mPGES-2, prostaglandin E synthase 2, GATE-binding factor 1, gamma-interferon-activated transcriptional element-binding factor 1, mPGE synthase-2, membrane-associated prostaglandin E synthase 2, microsomal prostaglandin E synthase-2, prostaglandin-H(2) E-isomerase
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:100
Background:
The protein encoded by this gene is a membrane-associated prostaglandin E synthase, which catalyzes the conversion of prostaglandin H2 to prostaglandin E2. This protein also has been shown to activate the transcription regulated by a gamma-interferon-activated transcription element (GATE). Multiple transcript variants have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 88-377 of human PTGES2 (NP_079348.1).
NCBI Gene ID #:
80142
NCBI Official Name:
prostaglandin E synthase 2
NCBI Official Symbol:
PTGES2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 35kDa
Purification:
Affinity purification
Research Area:
Signal Transduction
Swissprot #:
Q9H7Z7
User NOte:
Optimal dilutions for each application to be determined by the researcher.