ATRIP Antibody

ProSci
Product Code: PSI-22-734
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-734-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 2
Western blot analysis of extracts of various cell lines, using ATRIP antibody (22-734) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
2 / 2
Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug ATRIP antibody (22-734). Western blot was performed from the immunoprecipitate using ATRIP antibody (22-734) at a dilition of 1:1000.

Western blot analysis of extracts of various cell lines, using ATRIP antibody (22-734) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug ATRIP antibody (22-734). Western blot was performed from the immunoprecipitate using ATRIP antibody (22-734) at a dilition of 1:1000.

Further Information

Additional Names:
ATR-interacting protein, ATM and Rad3-related-interacting protein
Application Note:
WB: 1:500 - 1:2000

IP: 1:50 - 1:200
Background:
This gene encodes an essential component of the DNA damage checkpoint. The encoded protein binds to single-stranded DNA coated with replication protein A. The protein also interacts with the ataxia telangiectasia and Rad3 related protein kinase, resulting in its accumulation at intranuclear foci induced by DNA damage. Multiple transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human ATRIP (NP_569055.1).
NCBI Gene ID #:
84126
NCBI Official Name:
ATR interacting protein
NCBI Official Symbol:
ATRIP
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 86kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
Q8WXE1
User NOte:
Optimal dilutions for each application to be determined by the researcher.