UBASH3B Antibody

ProSci
Product Code: PSI-22-735
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-735-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using UBASH3B antibody (22-735) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
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Immunohistochemistry of paraffin-embedded rat spleen using UBASH3B antibody (22-735) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of A549 cells using UBASH3B antibody (22-735). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using UBASH3B antibody (22-735) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat spleen using UBASH3B antibody (22-735) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using UBASH3B antibody (22-735). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
STS-1, STS1, TULA-2, TULA2, p7ubiquitin-associated and SH3 domain-containing protein B, Cbl-interacting protein Sts-1, SH3 domain-containing 70 kDa protein, suppressor of T-cell receptor signaling 1, nm23-phosphorylated unknown substrate, T-cell ubiquitin ligand 2, cbl-interacting protein p7nm23-phosphorylated unknown substrate, suppressor of T-cell receptor signaling 1, tyrosine-protein phosphatase STS1/TULA2
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:100
Background:
This gene encodes a protein that contains a ubiquitin associated domain at the N-terminus, an SH3 domain, and a C-terminal domain with similarities to the catalytic motif of phosphoglycerate mutase. The encoded protein was found to inhibit endocytosis of epidermal growth factor receptor (EGFR) and platelet-derived growth factor receptor.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human UBASH3B (NP_116262.2).
NCBI Gene ID #:
84959
NCBI Official Name:
ubiquitin associated and SH3 domain containing B
NCBI Official Symbol:
UBASH3B
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 72kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
Q8TF42
User NOte:
Optimal dilutions for each application to be determined by the researcher.