TPH2 Antibody

ProSci
Product Code: PSI-22-739
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-739-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using TPH2 antibody (22-739) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 3
Immunohistochemistry of paraffin-embedded mouse brain using TPH2 antibody (22-739) at dilution of 1:200 (40x lens).
3 / 3
Immunofluorescence analysis of A549 cells using TPH2 antibody (22-739). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using TPH2 antibody (22-739) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded mouse brain using TPH2 antibody (22-739) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of A549 cells using TPH2 antibody (22-739). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
TPH2, NTPH, ADHD7
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:100
Background:
This gene encodes a member of the pterin-dependent aromatic acid hydroxylase family. The encoded protein catalyzes the first and rate limiting step in the biosynthesis of serotonin, an important hormone and neurotransmitter. Mutations in this gene may be associated with psychiatric diseases such as bipolar affective disorder and major depression.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human TPH2 (NP_775489.2).
NCBI Gene ID #:
121278
NCBI Official Name:
tryptophan hydroxylase 2
NCBI Official Symbol:
TPH2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 56kDa
Purification:
Affinity purification
Research Area:
Neuroscience
Swissprot #:
Q8IWU9
User NOte:
Optimal dilutions for each application to be determined by the researcher.