EPHA2 Antibody

ProSci
Product Code: PSI-22-763
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-763-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using EPHA2 antibody (22-763) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
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Immunohistochemistry of paraffin-embedded rat brain using EPHA2 Antibody (22-763) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using EPHA2 Antibody (22-763) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using EPHA2 antibody (22-763) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Immunohistochemistry of paraffin-embedded rat brain using EPHA2 Antibody (22-763) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using EPHA2 Antibody (22-763) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
EPHA2, CTPA, EPH receptor A2, Ephrin type-A receptor 2, Epithelial cell kinase, ECK, Sek-2, ARCC2, Soluble EPHA2 variant 1, CTPP1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. Mutations in this gene are the cause of certain genetically-related cataract disorders.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 847-976 of human EPHA2 (NP_004422.2).
NCBI Gene ID #:
1969
NCBI Official Name:
EPH receptor A2
NCBI Official Symbol:
EPHA2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 108kDa
Purification:
Affinity purification
Research Area:
Neuroscience, Signal Transduction
Swissprot #:
P29317
User NOte:
Optimal dilutions for each application to be determined by the researcher.