GTF2H3 Antibody

ProSci
Product Code: PSI-22-768
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-768-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 3
Western blot analysis of extracts of various cell lines, using GTF2H3 antibody (22-768) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
2 / 3
Immunofluorescence analysis of A549 cells using GTF2H3 antibody (22-768). Blue: DAPI for nuclear staining.
3 / 3
Immunoprecipitation analysis of 200ug extracts of HT-29 cells using 1ug GTF2H3 antibody (22-768). Western blot was performed from the immunoprecipitate using GTF2H3 antibody (22-768) at a dilition of 1:1000.

Western blot analysis of extracts of various cell lines, using GTF2H3 antibody (22-768) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Immunofluorescence analysis of A549 cells using GTF2H3 antibody (22-768). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of HT-29 cells using 1ug GTF2H3 antibody (22-768). Western blot was performed from the immunoprecipitate using GTF2H3 antibody (22-768) at a dilition of 1:1000.

Further Information

Additional Names:
GTranscription Factor Antibodies2H3, P34, BTF2, TFB4, TFIIH
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:100

IP: 1:50 - 1:200
Background:
This gene encodes a member of the TFB4 family. The encoded protein is a subunit of the core-TFIIH basal transcription factor and localizes to the nucleus. The encoded protein is involved in RNA transcription by RNA polymerase II and nucleotide excision repair and associates with the Cdk-activating kinase complex. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 14.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-308 of human GTF2H3 (NP_001507.2).
NCBI Gene ID #:
2967
NCBI Official Name:
general transcription factor IIH, polypeptide 3, 34kDa
NCBI Official Symbol:
GTF2H3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 36kDa
Purification:
Affinity purification
Research Area:
Transcription
Swissprot #:
Q13889
User NOte:
Optimal dilutions for each application to be determined by the researcher.