N6AMT1 Antibody

ProSci
Product Code: PSI-22-778
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-778-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using N6AMT1 antibody (22-778) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded rat brain using N6AMT1 Antibody (22-778) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human kidney using N6AMT1 Antibody (22-778) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of A549 cells using N6AMT1 antibody (22-778). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using N6AMT1 antibody (22-778) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat brain using N6AMT1 Antibody (22-778) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney using N6AMT1 Antibody (22-778) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using N6AMT1 antibody (22-778). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
C21orf127, HEMK2, MTQ2, N6AMT, PRED28, m.HsaHemK2P, methyltransferase N6AMT1, HemK methyltransferase family member 2, N-6 adenine-specific DNA methyltransferase 1 (putative), N6-DNA-methyltransferase, methylarsonite methyltransferase N6AMT1, protein N(5)-glutamine methyltransferase
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:100
Background:
This gene encodes an N(6)-adenine-specific DNA methyltransferase. The encoded enzyme may be involved in the methylation of release factor I during translation termination. This enzyme is also involved in converting the arsenic metabolite monomethylarsonous acid to the less toxic dimethylarsonic acid. Alternative splicing pf this gene results in multiple transcript variants. A related pseudogene has been identified on chromosome 11.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-186 of human N6AMT1 (NP_877426.3).
NCBI Gene ID #:
29104
NCBI Official Name:
N-6 adenine-specific DNA methyltransferase 1
NCBI Official Symbol:
N6AMT1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 23kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
Q9Y5N5
User NOte:
Optimal dilutions for each application to be determined by the researcher.