SYCE1 Antibody

ProSci
Product Code: PSI-22-791
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-791-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using SYCE1 antibody (22-791) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunofluorescence analysis of MCF-7 cells using SYCE1 antibody (22-791). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using SYCE1 antibody (22-791) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunofluorescence analysis of MCF-7 cells using SYCE1 antibody (22-791). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
C10orf94, CT76, POF12, SPGF15, synaptonemal complex central element protein 1, cancer/testis antigen 76, epididymis secretory sperm binding protein
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:100
Background:
This gene encodes a member of the synaptonemal complex, which links homologous chromosomes during prophase I of meiosis. The tripartite structure of the complex is highly conserved amongst metazoans. It consists of two lateral elements and a central region formed by transverse elements and a central element. The protein encoded by this gene localizes to the central element and is required for initiation and elongation of the synapsis. Allelic variants of this gene have been associated with premature ovarian failure and spermatogenic failure. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 132-351 of human SYCE1 (NP_001137236.1).
NCBI Gene ID #:
93426
NCBI Official Name:
synaptonemal complex central element protein 1
NCBI Official Symbol:
SYCE1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 40kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cell Cycle
Swissprot #:
Q8N0S2
User NOte:
Optimal dilutions for each application to be determined by the researcher.