POLD3 Antibody

ProSci
Product Code: PSI-22-808
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-808-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using POLD3 antibody (22-808) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 60s.
2 / 4
Immunohistochemistry of paraffin-embedded human oophoroma using POLD3 antibody (22-808) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using POLD3 antibody (22-808). Green:GFP-RNF168 fusion protein expression for DNA damage marker.Blue: DAPI for nuclear staining.RNF168(GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.
4 / 4
Immunofluorescence analysis of U2OS cells using POLD3 antibody (22-808).

Western blot analysis of extracts of various cell lines, using POLD3 antibody (22-808) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 60s.
Immunohistochemistry of paraffin-embedded human oophoroma using POLD3 antibody (22-808) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using POLD3 antibody (22-808). Green:GFP-RNF168 fusion protein expression for DNA damage marker.Blue: DAPI for nuclear staining.RNF168(GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.
Immunofluorescence analysis of U2OS cells using POLD3 antibody (22-808).

Further Information

Additional Names:
DNA polymerase delta subunit 3, DNA polymerase delta subunit p66, POLD3, KIAA0039
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:100
Background:
This gene encodes the 66-kDa subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. The encoded protein plays a role in regulating the activity of DNA polymerase delta through interactions with other subunits and the processivity cofactor proliferating cell nuclear antigen (PCNA). Alternatively spliced transcript variants have been observed for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 197-466 of human POLD3 (NP_006582.1).
NCBI Gene ID #:
10714
NCBI Official Name:
DNA polymerase delta subunit 3
NCBI Official Symbol:
POLD3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 51kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
Q15054
User NOte:
Optimal dilutions for each application to be determined by the researcher.