Acetyl-Histone H3-K9 pAb

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ProSci
Product Code: PSI-22-814
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-22-814-50uL50uL£505.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using Acetyl-Histone H3-K9 antibody (22-814) at 1:1000 dilution. Both NIH/3T3 cells and C6 cells were treated by TSA (1 uM) at 37°C for 18 hours.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
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Immunohistochemistry of paraffin-embedded human mammary cancer using Acetyl-Histone H3-K9 antibody (22-814) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded human colon using Acetyl-Histone H3-K9 antibody (22-814) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded rat ovary using Acetyl-Histone H3-K9 antibody (22-814) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using Acetyl-Histone H3-K9 antibody (22-814) at dilution of 1:200 (40x lens).
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Immunofluorescence analysis of HeLa cells using Acetyl-Histone H3-K9 antibody (22-814) at dilution of 1:100 (40x lens). HeLa cells were treated by TSA (1 uM) at 37°C for 18 hours (left). Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H3-K9 antibody (22-814) at dilution of 1:100 (40x lens). NIH/3T3 cells were treated by TSA (1 uM) at 37°C for 18 hours (left). Blue: DAPI for nuclear staining.
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Chromatin immunoprecipitation analysis of extracts of 293 cell line, using H3K9ac antibody (22-814) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
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Western blot analysis of extracts of various cell lines, using Acetyl-Histone H3-K9 antibody (22-814) at 1:1000 dilution. Both NIH/3T3 cells and C6 cells were treated by TSA (1 uM) at 37°C for 18 hours.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Immunohistochemistry of paraffin-embedded human mammary cancer using Acetyl-Histone H3-K9 antibody (22-814) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using Acetyl-Histone H3-K9 antibody (22-814) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat ovary using Acetyl-Histone H3-K9 antibody (22-814) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using Acetyl-Histone H3-K9 antibody (22-814) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of HeLa cells using Acetyl-Histone H3-K9 antibody (22-814) at dilution of 1:100 (40x lens). HeLa cells were treated by TSA (1 uM) at 37°C for 18 hours (left). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H3-K9 antibody (22-814) at dilution of 1:100 (40x lens). NIH/3T3 cells were treated by TSA (1 uM) at 37°C for 18 hours (left). Blue: DAPI for nuclear staining.
Chromatin immunoprecipitation analysis of extracts of 293 cell line, using H3K9ac antibody (22-814) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Documents

Further Information

Additional Names:
H3F3A, H3t, H3.4, H3/g, H3FT
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200

IP: 1:50 - 1:200

ChIP: 1:20 - 1:100

CHIP seq: 1:20 - 1:50
Background:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide of human Acetyl-Histone H3-K9
Modifications:
Acetylated
NCBI Gene ID #:
8290
NCBI Official Name:
histone cluster 3, H3
NCBI Official Symbol:
H3K9ac
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 16kDa
Purification:
Affinity purification
Research Area:
Signal Transduction
Swissprot #:
Q16695
User NOte:
Optimal dilutions for each application to be determined by the researcher.