Acetyl-Histone H4-K8 pAb

ProSci
Product Code: PSI-22-817
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-817-50uL50uL£505.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 7
Western blot analysis of extracts of various cell lines, using Acetyl-Histone H4-K8 antibody (22-817) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
2 / 7
Immunohistochemistry of paraffin-embedded rat ovary using H4K8ac antibody (22-817) at dilution of 1:100 (40x lens).
3 / 7
Immunohistochemistry of paraffin-embedded human breast using H4K8ac antibody (22-817) at dilution of 1:100 (40x lens).
4 / 7
Immunohistochemistry of paraffin-embedded human gastric cancer using H4K8ac antibody (22-817) at dilution of 1:100 (40x lens).
5 / 7
Immunohistochemistry of paraffin-embedded mouse brain using H4K8ac antibody (22-817) at dilution of 1:100 (40x lens).
6 / 7
Immunofluorescence analysis of HeLa cells using Acetyl-Histone H4-K8 antibody (22-817). Blue: DAPI for nuclear staining.
7 / 7
Chromatin immunoprecipitation analysis of extracts of 293 cell line, using H4K8ac antibody (22-817) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Western blot analysis of extracts of various cell lines, using Acetyl-Histone H4-K8 antibody (22-817) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat ovary using H4K8ac antibody (22-817) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast using H4K8ac antibody (22-817) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using H4K8ac antibody (22-817) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using H4K8ac antibody (22-817) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using Acetyl-Histone H4-K8 antibody (22-817). Blue: DAPI for nuclear staining.
Chromatin immunoprecipitation analysis of extracts of 293 cell line, using H4K8ac antibody (22-817) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Further Information

Additional Names:
H4/A, H4FA, HIST1H4B, H4/I, H4FI, HIST1H4C, H4/G, H4FG, HIST1H4D, H4/B, H4FB, HIST1H4E, H4/J, H4FJ, HIST1H4F, H4/C, H4FC, HIST1H4H, H4/H, H4FH, HIST1H4I, H4/M, H4FM, HIST1H4J, H4/E, H4FE, HIST1H4K, H4/D, H4FD, HIST1H4L, H4/K, H4FK, HIST2H4A, H4/N, H4F2, H4FN, HIST2H4, HIST2H4B, H4/O, H4FO, HIST4H4, Histone H4H4FA
Application Note:
WB: 1:1000 - 1:3000

IHC: 1:200 - 1:500

IF: 1:500 - 1:1000

IP: 1:200 - 1:500

CHIP: 1:20 - 1:50

CHIP seq: 1:20 - 1:50
Background:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide of human Acetyl-Histone H4-K8
Modifications:
Acetylated
NCBI Gene ID #:
8370
NCBI Official Name:
histone cluster 1, H4a
NCBI Official Symbol:
H4K8ac
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 11kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
P62805
User NOte:
Optimal dilutions for each application to be determined by the researcher.