CEBPG Antibody

ProSci
Product Code: PSI-22-831
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-831-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using CEBPG antibody (22-831) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded human colon carcinoma using CEBPG antibody (22-831) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human kidney using CEBPG antibody (22-831) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human kidney cancer using CEBPG antibody (22-831) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse liver using CEBPG antibody (22-831) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of A-549 cells using CEBPG antibody (22-831).

Western blot analysis of extracts of various cell lines, using CEBPG antibody (22-831) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human colon carcinoma using CEBPG antibody (22-831) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney using CEBPG antibody (22-831) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney cancer using CEBPG antibody (22-831) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using CEBPG antibody (22-831) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A-549 cells using CEBPG antibody (22-831).

Further Information

Additional Names:
CEBPG, GPE1BP, IG/EBP-1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:100
Background:
The C/EBP family of transcription factors regulates viral and cellular CCAAT/enhancer element-mediated transcription. C/EBP proteins contain the bZIP region, which is characterized by two motifs in the C-terminal half of the protein: a basic region involved in DNA binding and a leucine zipper motif involved in dimerization. The C/EBP family consist of several related proteins, C/EBP alpha, C/EBP beta, C/EBP gamma, and C/EBP delta, that form homodimers and that form heterodimers with each other. CCAAT/enhancer binding protein gamma may cooperate with Fos to bind PRE-I enhancer elements. Two transcript variants encoding the same protein have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human CEBPG (NP_001797.1).
NCBI Gene ID #:
1054
NCBI Official Name:
CCAAT/enhancer binding protein (C/EBP), gamma
NCBI Official Symbol:
CEBPG
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 16kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cell Cycle, Transcription
Swissprot #:
P53567
User NOte:
Optimal dilutions for each application to be determined by the researcher.