ING5 Antibody

ProSci
Product Code: PSI-22-837
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-837-50uL50uL£440.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using ING5 antibody (22-837) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 90s.
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Immunofluorescence analysis of MCF-7 cells using ING5 antibody (22-837). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using ING5 antibody (22-837) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 90s.
Immunofluorescence analysis of MCF-7 cells using ING5 antibody (22-837). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
Inhibitor of growth protein 5, p28ING5, ING5
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:100
Background:
This gene encodes a tumor suppressor protein that inhibits cell growth and induces apoptosis. This protein contains a PHD-type zinc finger. It interacts with tumor suppressor p53 and p300, a component of the histone acetyl transferase complex, suggesting a role in transcriptional regulation. Alternative splicing and the use of multiple promoters and 3' ends results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human ING5 (NP_115705.2).
NCBI Gene ID #:
84289
NCBI Official Name:
Inhibitor of growth protein 5
NCBI Official Symbol:
ING5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 28kDa
Purification:
Affinity purification
Research Area:
Cancer
Swissprot #:
Q8WYH8
User NOte:
Optimal dilutions for each application to be determined by the researcher.