NCBP2 Antibody

ProSci
Product Code: PSI-22-841
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-841-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using NCBP2 antibody (22-841) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded human colon using NCBP2 antibody (22-841) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded human prostate using NCBP2 antibody (22-841) at dilution of 1:200 (40x lens).
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Immunofluorescence analysis of MCF-7 cells using NCBP2 antibody (22-841). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using NCBP2 antibody (22-841) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human colon using NCBP2 antibody (22-841) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate using NCBP2 antibody (22-841) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of MCF-7 cells using NCBP2 antibody (22-841). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
CBC2, CBP2NIP1, PIG55, nuclear cap-binding protein subunit 2, 20 kDa nuclear cap-binding protein, NCBP 20 kDa subunit, NCBP interacting protein 1, cell proliferation-inducing gene 55 protein, nuclear cap binding protein subunit 2, 20kD, nuclear cap binding protein subunit 2, 20kDa
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:100
Background:
The product of this gene is a component of the nuclear cap-binding protein complex (CBC), which binds to the monomethylated 5' cap of nascent pre-mRNA in the nucleoplasm. The encoded protein has an RNP domain commonly found in RNA binding proteins, and contains the cap-binding activity. The CBC promotes pre-mRNA splicing, 3'-end processing, RNA nuclear export, and nonsense-mediated mRNA decay. Multiple transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-156 of human NCBP2 (NP_031388.2).
NCBI Gene ID #:
22916
NCBI Official Name:
nuclear cap binding protein subunit 2
NCBI Official Symbol:
NCBP2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 14kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
P52298
User NOte:
Optimal dilutions for each application to be determined by the researcher.