POU6F1 Antibody

ProSci
Product Code: PSI-22-845
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-845-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using POU6F1 antibody (22-845) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 90s.
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Immunofluorescence analysis of A-549 cells using POU6F1 antibody (22-845).

Western blot analysis of extracts of various cell lines, using POU6F1 antibody (22-845) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 90s.
Immunofluorescence analysis of A-549 cells using POU6F1 antibody (22-845).

Further Information

Additional Names:
POU6F1, BRN5, MPOU, TCFB1
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:100
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-301 of human POU6F1 (NP_002693.3).
NCBI Gene ID #:
5463
NCBI Official Name:
POU class 6 homeobox 1
NCBI Official Symbol:
POU6F1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 45kDa
Purification:
Affinity purification
Research Area:
Transcription
Swissprot #:
Q14863
User NOte:
Optimal dilutions for each application to be determined by the researcher.