PRKAG1 Antibody

ProSci
Product Code: PSI-22-846
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-846-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using PRKAG1 antibody (22-846) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 60s.
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Immunohistochemistry of paraffin-embedded human stomach using PRKAG1 antibody (22-846) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat brain using PRKAG1 antibody (22-846) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse kidney using PRKAG1 antibody (22-846) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of MCF-7 cells using PRKAG1 antibody (22-846). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using PRKAG1 antibody (22-846) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 60s.
Immunohistochemistry of paraffin-embedded human stomach using PRKAG1 antibody (22-846) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using PRKAG1 antibody (22-846) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using PRKAG1 antibody (22-846) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF-7 cells using PRKAG1 antibody (22-846). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
5'-AMP-activated protein kinase subunit gamma-1, AMPK gamma1, AMPK subunit gamma-1, AMPKg, PRKAG1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:100
Background:
The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit is one of the gamma regulatory subunits of AMPK. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-331 of human PRKAG1 (NP_002724.1).
NCBI Gene ID #:
5571
NCBI Official Name:
5'-AMP-activated protein kinase subunit gamma-1
NCBI Official Symbol:
PRKAG1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 36kDa/38kDa
Purification:
Affinity purification
Research Area:
Autophagy, Cancer, Cell Cycle, Signal Transduction
Swissprot #:
P54619
User NOte:
Optimal dilutions for each application to be determined by the researcher.