SETD8 Antibody

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ProSci
Product Code: PSI-22-850
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-850-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using SETD8 antibody (22-850) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 60s.
2 / 2
Immunofluorescence analysis of MCF-7 cells using SETD8 antibody (22-850).
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Western blot analysis of extracts of various cell lines, using SETD8 antibody (22-850) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 60s.
Immunofluorescence analysis of MCF-7 cells using SETD8 antibody (22-850).

Documents

Further Information

Additional Names:
SETD8, KMT5A, Lysine N-methyltransferase 5A, PR/SET07, PRSET7, SET8, H4-K20-HMTase SETD8, PR-Set7, SET07
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:100
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 73-352 of human SETD8 (NP_065115.3).
NCBI Gene ID #:
387893
NCBI Official Name:
SET domain containing (lysine methyltransferase) 8
NCBI Official Symbol:
SETD8
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 43kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
Q9NQR1
User NOte:
Optimal dilutions for each application to be determined by the researcher.