UBE2J2 Antibody

ProSci
Product Code: PSI-22-858
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-858-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using UBE2J2 antibody (22-858) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded rat liver using UBE2J2 antibody (22-858) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of MCF-7 cells using UBE2J2 antibody (22-858). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using UBE2J2 antibody (22-858) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat liver using UBE2J2 antibody (22-858) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF-7 cells using UBE2J2 antibody (22-858). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
UBE2J2, NCUBE2, PRO2121, NCUBE-2
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:100
Background:
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is located in the membrane of the endoplasmic reticulum. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human UBE2J2 (NP_477515.2).
NCBI Gene ID #:
118424
NCBI Official Name:
ubiquitin-conjugating enzyme E2, J2
NCBI Official Symbol:
UBE2J2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 29kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Neuroscience
Swissprot #:
Q8N2K1
User NOte:
Optimal dilutions for each application to be determined by the researcher.