HBA1 Antibody

ProSci
Product Code: PSI-22-863
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-863-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using HBA1 antibody (22-863) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 30s.
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Immunofluorescence analysis of MCF7 cells using HBA1 antibody (22-863). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using HBA1 antibody (22-863) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 30s.
Immunofluorescence analysis of MCF7 cells using HBA1 antibody (22-863). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
Hemoglobin subunit alpha, Alpha-globinHBH
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:100
Background:
The human alpha globin gene cluster located on chromosome 16 spans about 30 kb and includes seven loci: 5'- zeta - pseudozeta - mu - pseudoalpha-1 - alpha-2 - alpha-1 - theta - 3'. The alpha-2 (HBA2) and alpha-1 (HBA1) coding sequences are identical. These genes differ slightly over the 5' untranslated regions and the introns, but they differ significantly over the 3' untranslated regions. Two alpha chains plus two beta chains constitute HbA, which in normal adult life comprises about 97% of the total hemoglobin; alpha chains combine with delta chains to constitute HbA-2, which with HbF (fetal hemoglobin) makes up the remaining 3% of adult hemoglobin. Alpha thalassemias result from deletions of each of the alpha genes as well as deletions of both HBA2 and HBA1; some nondeletion alpha thalassemias have also been reported.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-142 of human HBA1 (NP_000508.1).
NCBI Gene ID #:
3039
NCBI Official Name:
hemoglobin, alpha 1
NCBI Official Symbol:
HBA1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 14kDa/25kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
P69905
User NOte:
Optimal dilutions for each application to be determined by the researcher.