PAX6 Antibody

ProSci
Product Code: PSI-22-869
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-869-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 2
Western blot analysis of extracts of various cell lines, using PAX6 antibody (22-869) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 30s.
2 / 2
Immunofluorescence analysis of A549 cells using PAX6 antibody (22-869). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using PAX6 antibody (22-869) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 30s.
Immunofluorescence analysis of A549 cells using PAX6 antibody (22-869). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
PAX6, AN, AN2, D11S812E, MGC17209, MGDA, WAGR
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200
Background:
This gene encodes a homeobox and paired domain-containing protein that binds DNA and functions as a regulator of transcription. Activity of this protein is key in the development of neural tissues, particularly the eye. This gene is regulated by multiple enhancers located up to hundreds of kilobases distant from this locus. Mutations in this gene or in the enhancer regions can cause ocular disorders such as aniridia and Peter's anomaly. Use of alternate promoters and alternative splicing result in multiple transcript variants encoding different isoforms.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human PAX6 (NP_000271.1).
NCBI Gene ID #:
5080
NCBI Official Name:
paired box 6
NCBI Official Symbol:
PAX6
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 47kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Neuroscience, Signal Transduction, Stem Cell, Transcription
Swissprot #:
P26367
User NOte:
Optimal dilutions for each application to be determined by the researcher.