PEX14 Antibody

ProSci
Product Code: PSI-22-871
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-871-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of mouse liver, using PEX14 antibody (22-871) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded human prostate cancer using PEX14 antibody (22-871) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human colon carcinoma using PEX14 antibody (22-871) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of A549 cells using PEX14 antibody (22-871). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of mouse liver, using PEX14 antibody (22-871) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human prostate cancer using PEX14 antibody (22-871) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using PEX14 antibody (22-871) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using PEX14 antibody (22-871). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
Peroxisomal membrane protein PEX14, PTS1 receptor-docking protein, Peroxin-14, Peroxisomal membrane anchor protein PEX14, PEX14
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes an essential component of the peroxisomal import machinery. The protein is integrated into peroxisome membranes with its C-terminus exposed to the cytosol, and interacts with the cytosolic receptor for proteins containing a PTS1 peroxisomal targeting signal. The protein also functions as a transcriptional corepressor and interacts with a histone deacetylase. A mutation in this gene results in one form of Zellweger syndrome.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 118-377 of human PEX14 (NP_004556.1).
NCBI Gene ID #:
5195
NCBI Official Name:
Peroxisomal membrane protein PEX14
NCBI Official Symbol:
PEX14
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 41kDa
Purification:
Affinity purification
Research Area:
Signal Transduction
Swissprot #:
O75381
User NOte:
Optimal dilutions for each application to be determined by the researcher.