PRDM5 Antibody

ProSci
Product Code: PSI-22-890
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-890-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using PRDM5 antibody (22-890) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 5
Immunohistochemistry of paraffin-embedded rat spleen using PRDM5 antibody (22-890) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse liver using PRDM5 antibody (22-890) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse spleen using PRDM5 antibody (22-890) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of A549 cells using PRDM5 antibody (22-890). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using PRDM5 antibody (22-890) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat spleen using PRDM5 antibody (22-890) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using PRDM5 antibody (22-890) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using PRDM5 antibody (22-890) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using PRDM5 antibody (22-890). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
PRDM5, PFM2, BCS2
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
The protein encoded by this gene is a transcription factor of the PR-domain protein family. It contains a PR-domain and multiple zinc finger motifs. Transcription factors of the PR-domain family are known to be involved in cell differentiation and tumorigenesis.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human PRDM5 (NP_061169.2).
NCBI Gene ID #:
11107
NCBI Official Name:
PR domain containing 5
NCBI Official Symbol:
PRDM5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 110kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
Q9NQX1
User NOte:
Optimal dilutions for each application to be determined by the researcher.