UBE2R2 Antibody

ProSci
Product Code: PSI-22-897
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-897-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using UBE2R2 antibody (22-897) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 30s.
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Immunohistochemistry of paraffin-embedded mouse cancer using UBE2R2 antibody (22-897) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of A549 cells using UBE2R2 antibody (22-897).

Western blot analysis of extracts of various cell lines, using UBE2R2 antibody (22-897) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded mouse cancer using UBE2R2 antibody (22-897) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using UBE2R2 antibody (22-897).

Further Information

Additional Names:
ubiquitin-conjugating enzyme UBC3B, ubiquitin-conjugating enzyme E2R 2, MGC10481, FLJ20419, CDC34B, UBC3B, UBE2R2
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
Protein kinase CK2 is a ubiquitous and pleiotropic Ser/Thr protein kinase involved in cell growth and transformation. This gene encodes a protein similar to the E2 ubiquitin conjugating enzyme UBC3/CDC34. Studies suggest that CK2-dependent phosphorylation of this ubiquitin-conjugating enzyme functions by regulating beta-TrCP substrate recognition and induces its interaction with beta-TrCP, enhancing beta-catenin degradation.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-238 of human UBE2R2 (NP_060281.2).
NCBI Gene ID #:
54926
NCBI Official Name:
ubiquitin-conjugating enzyme E2R 2
NCBI Official Symbol:
UBE2R2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 27kDa
Purification:
Affinity purification
Research Area:
Cell Cycle
Swissprot #:
Q712K3
User NOte:
Optimal dilutions for each application to be determined by the researcher.